Citrate Synthase Overexpression of <i>Pseudomonas putida</i> Increases Succinate Production from Acetate in Microaerobic Cultivation

Acetate is an end-product of anaerobic biodegradation and one the major metabolites microbial fermentation lingo-cellulosic hydrolysate. Recently, acetate has been highlighted as a feedstock to produce value-added chemicals. This study examined conversion succinate by citrate synthase (gltA)-overexpressed Pseudomonas putida under microaerobic conditions. The metabolism initiated with gltA enzyme, which converts acetyl-CoA citrate. gltA-overexpressing P. (gltA-KT) showed ∼50% improvement in production compared wild type. Under optimal pH 7.5, accumulation (4.73 ± 0.6 mM 36 h) was ∼400% higher than that Overall, overexpression alone resulted 9.5% maximum theoretical yield minimal medium sole carbon source. result shows important